Specialized hydrocarbonoclastic bacteria (SHCB), which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. These microorganisms, also called hydrocarbonoclastic microorganisms, . ” Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which. The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However.

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Different species of Marinobacter like alkaliphilus, aquaeolei, articus, hydrocarbonoclasticus, maritimus and squalenivorans are strictly HCB. Crude oil and hydrocarbon degrading strains of Rhodococcus rhodochrous isolated from soil and marine environments in Kuwait. This was essential for subsequent optical density measurements.

The results obtained is relatively lower than that of Abdullahi et al. Petroleum hydrocarbons are important energy resources used by industry and in our daily life, whose production contributes highly to environmental pollution.

Acinetobacter and Stenotrophomons were found hydrocarbonoclastc be the most abundant species characterized by a marked microdiversity as shown through ITS typing. Microbacterium jejuense Mycobacterium chlorophenolicum Alcanivorax dieselolei. Degradation of gasoline aromatic hydrocarbons by two N 2 -fixing soil bacteria. All the five bacteria isolates with potentials for hydrocarbon degradation did not exhibit lag phase of growth Fig 1 which might be attributed to their previous association with the hydrocarbon contaminated environment of the effluent drains from the refinery.

Droplets are eventually disintegrated to smaller size for their efficient utilization and uptake inside the cell. The results of this study are interesting from the view of recent studies in which hydrocarbons were shown to be utilized by conventional diazotrophic bacteria including Rhizobium 17Azotobacter 26and Klebsiella 1 Oil-utilizing bacterial species from oil-contaminated sites in Kuwait selected for this study.

Materials and Methods Bacterial isolates One hundred different species of oil-utilizing bacteria that have been hydrocarbonovlastic in our private culture collection were used in this study.

Most of the HCB are obligate hydrocarbonoclastic and occasionally utilize non-hydrocarbon substrate like hydrocarbonolastic and pyruvate. The pure isolated bacteria strains were identified morphologically and on the basis of their biochemical reactions in the Department of Biological Sciences, NDA. Table 3 Crude oil-consumption by bacterial species in the presence and absence of NaNO 3 in the medium and nitrogen gas in the culture top space.


Alcanivorax borkumensis and A. Sphingomonas dokdonensis 1 and Marinobacter M. PAHs present as natural constituents in fossils fuels are formed during the incomplete combustion of organic material and are therefore presents in relatively high concentrations in products of fossil fuels refining Wang et al.

Microbiology of oil-contaminated desert soils and coastal areas in the Arabian Gulf. They persist in nature for long period of time and cause hazardous effects on flora and fauna of terrestrial and aquatic ecosystems.

Petroleum microbiology – Wikipedia

Bioremediation is the use of biological agents such as microorganisms bacteria, fungi and algae and plants for the removal or detoxification of toxic substances in the environment. Physical and chemical approaches are capable of removing a broad spectrum of contaminants but the main disadvantage of these methods lie in the increased energy consumption and the need of additional chemicals Godheja et al.

Journal List Microbes Environ v. They utilize hydrocarbons as carbon and energy source for growth. This finding is similar to the observation made by Ojumuo et al. The mineral medium used was provided with 0.

Petroleum microbiology

More Bookmarks Digg etc. Support Center Support Center. Sorkhoh1 and Samir S. Growth curves were plotted. The predominant bacterial detected by both dependent and independent approaches were the Proteobacteria.

Some biosurfactants have low critical micelle concentrations CMCsa property which increases the apparent solubility of hydrocarbons by sequestering hydrophobic molecules into the centres of micelles.

February 8, ; Accepted: Other important characteristics of HCB are that they have very small genome, few rRNA operons and cytoplasm contains very small number of proteins. This hydrocarbonodlastic the largest oil spill of all time and indigenous petroleum microorganisms played a major role in petroleum degradation and cleanup. HCB are important constituents of oil degrading consortia and are usually present in hydrocarbon contaminated sites.


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We do not endorse these articles, we are neither affiliated with the authors of these articles nor responsible for their content. The vegetation-poor desert soil and Arabian Gulf water body hudrocarbonoclastic also poor in nitrogenous compounds.

In this work, a collection of strains, adapted to grow on minimal medium supplemented with crude oil, was obtained from contaminated sediments and seawater from a refinery harbor of the Bizerte coast in the North of Tunisia.

Emulsifiers produced by microbes can break up oil into smaller droplets, effectively increasing the hyddocarbonoclastic surface area. Phytoremediation for oily desert soil. Table 3 shows the quantitative data of oil consumption by the individual bacterial species in the presence and absence of NaNO 3 in the medium and nitrogen gas in the head space.

Most 12 species of these species belonged to Alphaproteobacteria and Gammaproteobacteriawhich was consistent with previous findings on hydrocarbonoclastic bacteria in contaminated sediments and seawater from a refinery harbor in the north of Tunisia For example, bacteria may be used to identify a pollutant by monitoring their response to the specific chemical.

Survey of petroleum- degrading bacteria in coastal waters of Sunderban Biosphere Reserve. Not only bacteria but some fungi and yeasts are also known to be hydrocarbonoclastic.

Kuwait was suitable for this study because it is a hydrocabronoclastic approximately18, km 2but major oil-producing country. Proc Roy Soc London. Therefore, these isolates can be referred to as hydrocabonoclastic bacteria because of their possession of catabolic enzymes for hydrocarbon degradation.

The results of sequencing of the nifH gene bands in Fig. When further supplied with sufficient limiting nutrients such as nitrogen and phosphor, it grows and produces surfactant glucolipids to help reduce surface water tension and enhance hydrocarbon uptake. Nesiotobacter exalbescens Acinetobacter junii Arthrobacter globiformis.

COD reduction by bacterial isolates from Refinery effluent.