Mangostin is a natural xanthonoid, a type of organic compound isolated from various parts of the mangosteen tree (Garcinia mangostana). It is a yellow. The anticancer activity is induced by xanthones such as alpha-, beta-, and gamma-mangostin which are major constituents of the pericarp of mangosteen fruits. In order to obtain the biological active compound, α-mangostin, from the traditional native mangosteen (Garcinia mangostana L.), an extraction.

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The cells treated with Bax and Bad are pro-apoptotic factors that promote apoptosis, whereas Bcl-2 is an mangosstin factor 31 Membranes were incubated with anti-caspase-3, anti-caspase-9 and anti-PARP antibodies. Introduction Cancer is one of the most life-threatening diseases worldwide, and the incidence and mortality of cancer in Korea have continuously increased due to various acquired risk factors, including a Western diet and environmental factors 1.

Mangostin | C24H26O6 – PubChem

Int J Mol Sci. Bao Q and Shi Y: The results of immunohistochemical analysis of cleaved caspase-3 were similar to those of western blot analysis Fig. International Journal of Molecular Medicine.

The apoptotic cells were analyzed quantitatively by flow cytometry to determine whether the morphological changes alpah in the DAPI-stained chromosomes were caused by apoptosis. A marked difference between the treated and control groups was observed beginning on day 8 following treatment Fig. A Ki antibody was used to differentiate nuclei in proliferating cells G1, S, G2 and M phases from those in resting cells, as previously described The expression of c-myc also decreased.


The mice in the control group received the vehicle 0. Fucoidan induces apoptosis of human HS-sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways.

Sign up for eToc alerts. Mice were divided into 3 groups: Thus, as regards the development of particular anticancer drugs and preventive medications, the extent that a substance affects cancer cell mangostib progression should be identified.

alpha-mangostin (CHEBI)

The percentage of apoptotic cells in the untreated control group was DAPI staining was performed to identify the morphological changes associated with nuclear and chromosomal condensation. Bcl-2 family proteins control membrane permeability and are located in the mitochondrial membrane or move to the mitochondrial membrane to induce apoptotic cell death Following incubation with the primary antibodies, the membranes were incubated with HRP-conjugated goat anti-rabbit IgG secondary antibodies for 2 h at room temperature with gentle shaking.

All animal experiments were performed following the approval of the Institutional Animal Care and Use Committee according to the guidelines of Kongju National University. Medicinal properties of mangosteen Garcinia mangostana.

Caspases are further classified into initiator and effector caspases. Statistical significance was determined by the Dunnett’s t-test. Caspase activation and PARP cleavage are typical characteristics of apoptosis Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki International Journal of Molecular Medicine, 37, Tumor weights in the experimental nude mice were also determined to be 0.


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Apoptotic bodies were stained with DAPI. It is therefore necessary to find naturally-derived substances to serve as anticancer drugs that are capable of specifically targeting cancer cells, with limited side-effects and potent anticancer effects.

Chemical constituents of Garcinia mangostana. Immunohistochemical analysis was also performed to examine the expression of Ki, the protein responsible for the rate of tumor mangoxtin Fig.

To identify Ki protein expression, immunohistochemistry was performed as described in the Material and methods. Oral cancer and precancerous lesions. Thus far, various xanthones have been found in fruit, fruit skin, tree bark, moss and mold, and approximately alph different xanthones have been found in the mangosteen fruit Targeting pMAPK in the ischaemic heart: The percentage of viable cells relative to the untreated control cells was estimated.

B The sub-G1 fraction was assessed by PI staining and flow cytometry.